Calibration of UV by Control of Wavelength with Demo image

Calibration of UV by Control  of  Wavelength

CONTROL OF WAVELENGTH

Holmium Oxide Solution

Weight accurately 1.0 gm of Holmium Oxide and dissolve it in 1.4 M Perchloric acid solution. Make up to 25 ml with the same solvent.

Go to spectrum mode

  • Make sure that Reference and sample cell holder is empty and press button for baseline correction(200 to 700).
  • Then keep the Holmium oxide filter in the sample cell holder
  • Close the cell compartment
  • Scan in the range of 200 to 700 nm for spectrum.
  • After scanned (go to Operations--click Peak Pick)
  • The spectrum shall show maximum at the following wavelength
  • And record the observed maxima.

Maxima at Wavelength (nm)Limit (nm)
241.15240.15 to 242.15
287.15286.15 to 288.15
361.5360.5 to 362.5
536.3533.3 to 539.3


Calibration of UV by Control  of  Wavelength with Demo image
CONTROL OF WAVELENGTH(UV calibration)


Calibration of UV by Control  of  Wavelength with Demo image
CONTROL OF WAVELENGTH ( UV calibration )






If calibration is satisfactory then affix the status label on it with the next due calibration date.

Frequency

Once in a 3 month

REFERENCE

Indian Pharmacopoeia/ British Pharmacopoeia/ US Pharmacopoeia / TGA Appendix D SOP : Operation Of Spectro Photometer