LIMIT OF STRAY LIGHT(UV calibration)

LIMIT OF STRAY LIGHT


Weight accurately 1.2 gm of Potassium chloride (previously dried at 105oC for two hours) and dissolve & make up to 100 ml with distiled water.

Go to Photometric mode


1. press Ctr+M
2. Add wavelenth 200 nm{Select RAW DATA}
3. Auto zero
4. Then keep the Potassium chloride Solution in the sample cell holder & using water as a blank 
5. Close the cell compartment
6. click Start
7. And record the observed Abs. 

Acceptance criteria:-

Absorbance is greater than 2.

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