LIMIT OF STRAY LIGHT(UV calibration)

LIMIT OF STRAY LIGHT

Weight accurately 1.2 gm of Potassium chloride (previously dried at 105^oC for two hours) and dissolve & make up to 100 ml with distiled water.

Go to Photometric mode

1. press Ctr+M

2. Add wavelenth 200 nm{Select RAW DATA}

3. Auto zero

4. Then keep the Potassium chloride Solution in the sample cell holder & using water as a blank 

5. Close the cell compartment

6. click Start

7. And record the observed Abs. 

Acceptance criteria:-

Absorbance is greater than 2.