Limit of Stray Light KCL ( Calibration of UV-Spectrometer ) - KCL

Calibration of UV by Limit of Stray Light KCL

Limit of Stray Light

Potassium Chloride Solution

Weight accurately 1.2 gm of Potassium chloride (previously dried at 105 C for two hours) and dissolve & make up to 100 ml with distilled water.

Go to Photo metric mode

  • Press Ctr + M
  • Add wavelength 200 nm {Select RAW DATA}
  • Auto zero
  • Then keep the Potassium chloride Solution in the sample cell holder & using water as a blank Close the cell compartment
  • Click Start
  • And record the observed Abs. 

Acceptance criteria:-

Absorbance is greater than 2.

sceenshot of  Limit of Stray Light

If calibration is satisfactory then affix the status label on it with the next due calibration date.


Once in a 3 month


Indian Pharmacopoeia/ British Pharmacopoeia/ US Pharmacopoeia / TGA Appendix D SOP : Operation Of Spectro Photometer

Post a Comment