Calibration of UV by Limit of Stray Light KCL ( Screenshot )

Calibration of UV by Limit of Stray Light KCL

Calibration of UV by Limit of Stray Light

Potassium Chloride Solution

Weight accurately 1.2 gm of Potassium chloride (previously dried at 105 C for two hours) and dissolve & make up to 100 ml with distilled water.


Go to Photo metric mode

  • Press Ctr+M
  • Add wavelength 200 nm {Select RAW DATA}
  • Auto zero
  • Then keep the Potassium chloride Solution in the sample cell holder & using water as a blank Close the cell compartment
  • Click Start
  • And record the observed Abs. 

Acceptance criteria:-

Absorbance is greater than 2.


sceenshot of  Limit of Stray Light



If calibration is satisfactory then affix the status label on it with the next due calibration date.

Frequency

Once in a 3 month

REFERENCE

Indian Pharmacopoeia/ British Pharmacopoeia/ US Pharmacopoeia / TGA Appendix D SOP : Operation Of Spectro Photometer



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