Fat Content/Fatty Matter
Apparatus
- Drying oven – 105° C
- Weighing Balance
- Flat RB flask-250ml
- Soxhlet Extractors- 250 ml
- Desiccators
- Thimbles
Reagent
- Dichloromethane
Procedure
Dry the Flat RB flask for half an hour at 105° C. cool for about 20min in Desiccators and weight. Weight 2 gm of sample. Place the sample in a thimble in a Soxhlet extractor with Dichloromethane solvent .siphons at least six times per hour for three hours. Evaporate the extract carefully to dryness in the RB flask on a stream bath. Further dry the contents in the drying oven at 105° C to constant mass. Cool in a desiccators and weight.
Calculation
Fat content (%) = (c-b) * 100 / a
Where
a mass of sample
b Oven dry-mass of Flat RB flask
c Oven dry-mass of Flat RB flask with exact
REFERANCE: – IS 199-1989
