Limit of Stray Light KCL ( Calibration of UV-Spectrometer ) – KCL

Potassium Chloride Solution

Weight accurately 1.2 gm of Potassium chloride (previously dried at 105 
C for two hours) and dissolve & make up to 100 ml with distilled water.

Go to Photo metric mode

  • Press Ctr + M

  • Add wavelength 200 nm {Select RAW DATA}

  • Auto zero

  • Then keep the Potassium chloride Solution in the sample cell holder & using water as a blank Close the cell compartment

  • Click Start

  • And record the observed Abs. 

Acceptance criteria:-

Absorbance is greater than 2.
sceenshot of  Limit of Stray Light

If calibration is satisfactory then affix the status label on it with the next due calibration date.


Once in a 3 month


Indian Pharmacopoeia/ British Pharmacopoeia/ US Pharmacopoeia / TGA Appendix D SOP : Operation Of Spectro Photometer

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