Photometric Accuracy ( Calibration of UV-Spectrometer ) – KNO3

Calibration of UV-Spectrometer Photometric Accuracy

Potassium Nitrate  solution

Prepare 1.065 % w/v. 0.710 % w/v, 0.355 % w/v solution of Potassium Nitrate (Previously dried at 130 C) .



Go to Photo metric mode

  • Press Ctr + M
  • Add wavelength 302 nm { Select RAW DATA }
  • Auto zero
  • Then keep the Potassium Nitrate Solution in the sample cell holder & using water as a blank. 
  • Close the cell compartment.
  • Click Start
  • And record the observed Abs. 


Acceptance criteria:-

Concentration % w/v Absorbance limit
1.065 % 0.741 to 0.761
0.710 % 0.490 to 0.510
0.355 % 0.240 to 0.260



Screenshot of UV Photometric Accuracy
Screenshot of  UV Photometric Accuracy



If calibration is satisfactory then affix the status label on it with the next due calibration date.

Frequency

Once in a 3 month

REFERENCE

Indian Pharmacopoeia/ British Pharmacopoeia/ US Pharmacopoeia / TGA Appendix D SOP : Operation Of Spectro Photometer


Author: Aman Kumar Sah

I am a professional blogger from India having rich experience in the Consumer Services Industry (Skilled in Textile & Leather) field.

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