Resolution power ( Calibration of UV-Spectrometer ) – Toluene

Calibration of by UV Resolution power Toluene

0.02 % ( v/v ) Toluene solution

Take 20 μl of Toluene and dissolve & make up to 100 ml with Hexane.

Go to Spectrum mode

  • Make sure that Reference and sample cell holder is empty and press button for baseline correction(230 to 330). 

Note:-(before use check the hexane for transmittance, using water as a blank between 230 nm to 330 nm & use only if transmittance is not less than 97%).

  • Then keep the 0.02 % Toluene solution in the sample cell holder & using Hexane as a blank 
  • Close the cell compartment 
  • Scan in the range of 230 to 330 nm for spectrum.
  • And record the observed Abs. 

Acceptance criteria:-

Ratio = Absorbance at 269 nm / Absorbance at 266 nm = Not less than 1.5

Screenshot of Resolution power

Screenshot of Resolution power

More Topic related to Uv calibration

If calibration is satisfactory then affix the status label on it with the next due calibration date.


Once in a 3 month


Indian Pharmacopoeia/ British Pharmacopoeia/ US Pharmacopoeia / TGA Appendix D SOP : Operation Of Spectro Photometer

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